I am trying to use DESEQ2 dor DGE analysis. I am using paired datasets uploaded from GEO, aligned with Hisat2 and counted with HTseq-counts. I am selecting the input files, Have the source set as HTseq-counts, but I keep getting the error below.
In Sys.setlocale(“LC_MESSAGES”, “en_US.UTF-8”) :
OS reports request to set locale to “en_US.UTF-8” cannot be honored
.rowNamesDF<-(x, value = value) :
duplicate ‘row.names’ are not allowed
Calls: rownames<- … row.names<- → row.names<-.data.frame → .rowNamesDF<-
non-unique value when setting ‘row.names’: ‘SRR16947329’
I am not manipulating the dataframe from the counting program at all prior to input. It has worked before. At a loss on what to correct.
Thanks for any help!