Hi @jennaj,
thanks for your help.
I’m working on the Galaxy Europe server: Galaxy
My issue is that I’m trying to perform an assembly-based functional annotation — starting from scaffolds, not from raw reads, from my metagenomic sequencing.
The workflow I was following is:
Prodigal (gene prediction) → DIAMOND (protein annotation using UniRef90 database) → HUMAnN (metabolic pathway reconstruction) → UniRef to KEGG KO conversion.
The problem is that I cannot find the UniRef90 database among the available reference databases in the DIAMOND tool on Galaxy.
I understand that a read-based profiling directly with HUMAnN is simpler, but since I already have assembled scaffolds, I would like to perform a more targeted assembly-based analysis.
Could you please tell me if the UniRef90 database can be made available for DIAMOND on Galaxy Europe, or who I should contact to request it?
Thank you very much for your time and support.
Best regards,
Elena