Hi @Abhisek_Dey
Which protocol are you following? These details will help:
- tutorial link (if following one)
- workflow link (if using one)
- full name/version of the tool that produced this error (top of the tool form)
- how this job was set up – find this information on the job information Details page (using the i-icon)
If you share links for the item 1 above then a link to the shared history that would include everything else. Or, you can explain directly, maybe with screenshots. How to. → How to get faster help with your question
Then, as a guess for why this message was reported
It seems the tool is reporting that it expected replicates for one of the batches. Meaning, at least two samples per batch. This seems important for a batch correction step, any tool, otherwise, there is nothing to compare within a “batch” using just one sample.
And for the larger question here
I’m not sure if you need to reorganize your data or if this is all you have, but if you don’t have two samples then batch correction at a minimum is likely not possible. Whether the remainder of the protocol is possible is hard to answer without seeing more details. You might need to find an example that sets up the data the way you plan to and then follow the modified protocol. This could involve parameter differences, or different tools. We can try to point you to the scientists who can answer the larger questions if you share where the protocol was sourced from originally.
Let’s start there, thanks! 