Hi @lore77
Please post a question just one time, we’ll see it. I’m consolidating your other question into this topic. Xref → Getting data from tutorial 16s Microbial analysis with Mothur
For your question, please explain a bit more and we can help.
- Tutorial link. Which tutorial are you following, and at what step? You can copy/paste the link to the tutorial step back here. There are two associated with this tool, so being specific will help us to clarify where things are going wrong (and fix on our side if something is not clear).
- Shared History link. What did your error look like exactly? Have you tried a rerun? You can generate and post back a share link to your history for troubleshooting help. See the banner at this forum for how to do that, or here directly. → How to get faster help with your question
Loading data from a tutorial should definitely work, so it might a problem in how that was done with the Upload tool. We can help to clarify, and even adjust the tutorial instructions to make it clearer for everyone.
Running a tool on your own data, that later errors, can be due to a much larger range of reasons. It could a scientific problem in the data, or how that data was organized, or even parameter choice issue. Solving that is what most questions at this forum are about. Bioinformatics can be quite complicated, even when doing your research through a web interface! 
Let’s start there! Once you share everything we can help to exactly address the problems. Let’s start with the tutorial part first, since creating your “answer key” history is a great way to learn how the processing works before attempting to do this with your own data. Thanks! 