Welcome @OzGuo
These are strange results!
Sometimes the SRA can get busy at times and reject connections with odd reasons (everyone is impacted, not just Galaxy users).
To try to reproduce this, I’ve started up two tests in this history to retrieve these GEO fastq reads for → https://www.ncbi.nlm.nih.gov/sra/?term=SRR2033948
I’m 1) getting the reads directly with the Faster Download and Extract Reads in FASTQ format from NCBI SRA tool using the accession and also 2) pulled in the sra archive to get the reads that way instead (using the same tool to read the archive). Let’s see if I can reproduce the problem.
It seems that both of those methods worked now! Would you like to try again as well? I’ll leave this history shared in case you would like to examine the methods closer.
Please let us know if you are able to get a successful run or not! ![]()