GTDB-Tk database unavailable on usegalaxy.org (Release 232)

Ghadi · November 11, 2026, 5:30pm

Hello Galaxy Support Team,

I am following the Galaxy Training Network tutorial “Build, Refine, and Annotate Metagenome-Assembled Genomes (MAGs)” on usegalaxy.org.

I have successfully completed genome assembly, binning, bin refinement, and dereplication. My final dataset collection is 1058: dereplicated_genomes, containing 15 FASTA genomes.

However, when I open GTDB-Tk Classify genomes (Galaxy tool version 2.8.0+galaxy0), the required GTDB-Tk database field displays:

“No options available.”

The tool also states that this version requires GTDB Release 232 and advises contacting the service administrator if the database is unavailable.

Could you please confirm whether GTDB Release 232 is available on usegalaxy.org, or advise how I can run GTDB-Tk on my existing dereplicated genomes without repeating the upstream analyses?

Galaxy server: https://usegalaxy.org/

Training tutorial: Hands-on: Building and Annotating Metagenome-Assembled Genomes (MAGs) from Short Metagenomics Paired Reads / Building and Annotating Metagenome-Assembled Genomes (MAGs) from Short Metagenomics Paired Reads / Microbiome

Thank you for your help.