Hello, I am doing 16s rRNA (V5-V7 region) metagenomics analysis. I downloaded SILVA_138.2_SSURef_NR99_tax_silva.fasta.gz from ( https://www.arb-silva.de/fileadmin/silva_databases/release_138_2/Exports/SILVA_138.2_SSURef_NR99_tax_silva.fasta.gz ). Uploaded its FASTA file as a reference in align.seqs bu…

Hi @Ahmer It sounds like you are getting a scientific result, and that is probably because of the difference in your target region needs.You can get the file for the appropriate region, and use the tutorial file as an example of the expected data format. Meaning: get the correct file for scientifi…

Welcome, @Ahmer Your reference file is the correct data to use but it must be in an uncompressed format. You can uncompress it using the convert function under the pencil icon. FAQ: Converting the file format . Then, to test if the problem is with the database options, you could grab a copy of the …

Hi Jenny, Thank you for your response. I am currently trying to use the reference data file in FASTA format. However, regarding the database option you’re suggesting, it seems to be tailored for the V4 region. Since my data pertains to the V5-V7 regions, there is a difference in base pair sizes. I…

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How to add SILVA_138.2 latets as a reference to align.seqs tool in GALAXY Server?

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