how to make a BED file

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1

Dear Sir/Madam,

I setup a bed file (see below), but it does not work. I wonder how to correct it? Thanks.

HPV16 1024 1043 HPV_1_LEFT 1 + GCDCARGGDCAYAAYAATGG
HPV16 1183 1207 HPV_1_RIGHT 1 - GAAAAATAAACTGTAAATCATATTC

Best regards,
Chen

2

Hello @huiping

The specification for BED file content is here: Genome Browser FAQ

And this tutorial covers common data manipulation how-to: Data Manipulation Olympics

XRef: primer trim by galaxy tools - #5 by wm75