How to use various nanopore fastq files?

Hi, I wanted to run a workflow named “Genome assembly and gene annotation” originally by Javier Rueda. I have about 150 nanopore fastq files. I put them all in a collection and want to run the workflow but it says “no compatible datasets available”. If I do a concatenate with all these files, I can run the workflow, but I learned that I should not concatenate the files? what is correct? how can I run the workflow?

Welcome @Andrea_Frei

We can probably help! The workflow seems to be expecting a different format or organization of the data. And my guess is that you are correct, concatenating isn’t the right solution. Instead you want to make an adjustment to have the workflow process and input collection instead of an individual dataset. This is definitely possible!

Would you like to share a link to the workflow and your history? This is how to share the history FAQ: Sharing your History and workflows are the similiar, find the Share button on the workflow card. You can post both links back here in a reply, then unshare when we are done. If the workflow is associated with a tutorial, you can link that back here for more context.

Let’s start there! :slight_smile:



:graduation_cap: GTN Tutorials

As a guess, is this the tutorial/workflow?

Others related to your question include: