Hello Galaxy team,
I would like to perform image correlation in GALAXY EU while allowing translation, rotation, and uniform scaling, but preventing any aspect ratio distortion (i.e., no non-uniform scaling or shear).
This is for two images of different resolution/size, for example a microscopic image and a MALDI-MSI image of the same tissue section. I noticed that the landmark registration tool can be used for alignment, but it appears to introduce changes in aspect ratio, and I cannot find an option to disable this distortion. I may be overlooking something, but I could not identify any setting that enforces preservation of aspect ratio—even though the GALAXY AI suggested this should be possible.
Is image correlation in GALAXY EU possible under these constraints (similarity transformation only)? If yes, could you please suggest which tools or workflows would be appropriate?
Thank you in advance for your help.