Meryl troubleshooting

[jennaj]

Hi @Lorenzo

Your job at the UseGalaxy.org.au server used a tool version that includes the prior fix. Maybe something else is going wrong.

@Lorenzo

Input data is PacBio HiFi ccs.bam file

The tool is expecting reads as an input, not a BAM. Even if the BAM includes only-reads (not a mapping result), those reads should be extracted into fastq/fasta formatted datasets for this tool to understand the input. VGP assembly pipeline

Tools filter for appropriate inputs based on the datatype. Dragging and dropping inputs can avoid that check but it is not recommended, and expect errors if you try.

@jennaj

if you are ever not sure what datatype(s) a tool accepts as input, any tool not just those that accept reads, try this:

1. Create a new empty history
2. Load up the tool form
3. Review the input datatypes listed in the tool form’s input area
4. Note: A tool could have more than one input area and this works for all user-supplied inputs from the history.

Example

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If this doesn’t address the problem, would you please generate and post back a shared history link Sharing your History? We can review in more detail that way. Or, you can post back more details just about the input 5 : Merged meryl 1 since that seems to be at least part of the problem.