Hello,
I successfully created a database using the makeblastdb command and ran blastx, but it did not return any taxonomic or gene information. Below is an example of the input fasta file I gave makeblastdb (should I have used -parse_ids?) and query fasta:
db fasta (downloaded directly from NCBI):
AVN99676.1 histone H3, partial [Turbinaria sp. (in: Phaeophyceae)]
KQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVLALQEASEAYLVGLFEDTNLCAIHAKRVT
query fasta:
Contig121
agtaacagcagcaaggcaggagggtcgaggcctggtgagcctgagtacctgtgcaatgagtgcgggctgggcttcgggagcgtgccggagctgcagatgcatatggccaggaagacggcttggtcgaaccaggggctggtggggtgcagggtcagctaccaagagcacaaacgaaaacgaaaacaaagccatgtaaatgtgcagtaccacaagtccgggaagcactgcgtggagtaccgcaggtctggggagaaacggtgggtgaatatgctgcggacggccttctacatcgtggagcgcgcgcagcccaacgggggggaagtgaaggagatggagccggacccgacggaaggattggccccgatcgagaagtggcactacgaggaggacatctcaccggagttctgcgtggcgttatccctcctacaccgagcctacggctcccgggttcaggagaccgggcataagaccgtgggtcatacctgcgtcacggatcgggacaaagtaacggcccgacaggtcaagggttctctgctgtacggagagcttcttcccaggggggtgaacaaggcgatggggtcaaaacacctaaacgccgcgaaggcttcgacggtgtacgatctggggatgggcactggcaaagtggcgatgcaggtgttcctgcagtacaccaatctcacgagggtgtacggcatcgagctgtccaaggcccgcttcgagctgggagaggcggg
Returned blast results:
Contig121 CBJ26512.1 84.649 228 34 1 46 729 145 371 5.24e-137 404 emb|CBJ26512.1| 1037 193 205 1 89.91 1 0 YLCNECGLGFGSVPELQMHMARKTAWSNQGLVGCRVSYQEHKRKRKQSHVNVQYHKSGKHCVEYRRSGEKRWVNMLRTAFYIVERAQPNGGEVKEMEPDPTEGLAPIEKWHYEEDISPEFCVALSLLHRAYGSRVQETGHKTVGHTCVT
Thank you for your help!
Sincerely,
Rachael