Welcome @Peter_Janampa
It seems this prior issue is pending a correction. → Problem on Picrust2 webtool, even with tutorial files
I’ve updated the ticket to include a link to this topic and the updated testing history originally used. You are welcome to review the history for a format example! For the technical specification, you can start here http://biom-format.org/.
Any tool in Galaxy that creates the same format will be following the official specification above. To find tools to create, or manipulate biom files, including the shared.biom format, search the tool panel with the keyword “biom”.
Then, “shared” files in the context of the Mothur tool suite have a specific format. Many examples are in the tutorial here, and I’ve linked place where the plain format is include along with some explaintations.
- Hands-on: 16S Microbial Analysis with mothur (extended) / 16S Microbial Analysis with mothur (extended) / Microbiome
- Output of that tutorial’s workflow run on the tutorial data → https://usegalaxy.org/workflows/invocations/1641bb092161e9ab
- click into dataset 124 for a
mothur.sharedfile. It is a special tabular format with headers and columns of datapoints. A tools like Make.biom and Biom.info are packing and unpacking information from the original underlyingbiomdata.
Let’s see if the EU administrators can offer some feedback at their ticket! Even correct formats are failing right now. Hopefully that can be adjusted. 