Thanks, I saw your report plus can reproduce the “fails immediately” issue with test data across a set of specific tools.
There is a problem with one of our dedicated clusters. Unicycler, Spades, plus a few other tools are impacted. Our administrator is working on it. We’ll post back an update tomorrow, even if not fully resolved yet.
If your work is urgent, you might want to try the usegalaxy.eu server as a short-term alternative for now. Most public Galaxy servers are very very busy, so using more than one is common. Usage terms are one account per person per public Galaxy server – so it is fine to use both, plus any others that host the tools you are interested in: https://galaxyproject.org/use/
All the feedback appreciated!