VGP - Workflow 1: K-mer Profiling - Need 2 PacBio Hifi Files But I Only Have 1

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Hello!

I’m an undergrad student, so please excuse me if there is a simple solution to this I’m not seeing.

I’m trying to run the first workflow–K-mer profiling–in the VGP-Galaxy Assembly Workflows Series by following this tutorial. The very first step of the K-mer profile analysis workflow says that it needs HiFi reads as a collection, but I only have one Hifi for my species. I can’t figure out how I am supposed to even start if I can’t get past the first step.

Any help would be much appreciated!!

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Hi @adswann

To make sure I understand: a workflow that you want to use expects a collection input but you only have one sample. Yes?

If so, you can create a collection with just one sample. Follow the same building method.

Hope this helps and let us know if this works out or if you need more help. :slight_smile:

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Hi!

Thank you, that did work! I don’t know why I didn’t think to try making a collection with just the one file… :sweat_smile: I appreciate the help!!

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No worries! Thinking about the big details and getting stuck on the small ones is sort of an occupational hazard in bioinformatics! Any questions are welcome here, no judgement. :slight_smile: