How do I concatenate multiple fastq.gz files into a single fastq.gz file in Galaxy? I am assembling a bacterial genome with oxford nanopore minION sequences, and I have 68 fastq.gz files that are all under the same barcode. Under the “What type of data do you wish to concatenate?” menu, i have use…

Welcome @albert_burkle To make sure I understand: you currently have a paired end collection of files in fastq.gz format. Each file is one or more sequences. You want concatenate these into a single pair of files, each with multiple sequences. If that is all true, then the Collection Operation too…

Concatenate multiple datasets tool; combining all fastq.gz files under a single barcode to one fastq.gz file

jennaj July 22, 2025, 10:10pm 7

This includes any prior issues with fastq.gz uncompression failures (any tool).

Topic		Replies	Views
How to merge multiple fastq.gz files from one sample into one fastq.gz file usegalaxy.eu support gtn-tutorial , workflow , collections	3	3773	February 27, 2023
Tool for merging 2x single-read illumina sequencing files (fastq) into one? usegalaxy.org support text-manipulation , macs2 , fastqsanger , epigenetics	7	683	November 5, 2021
how to create a paired dataset collection from the files which are already paired collections	11	1142	July 28, 2023
Paird-end Fastq-dump Manipulation - Fastq De-Interlacer	3	2528	May 7, 2019
concatenate (corresponding) data sets in collections tool-search	1	821	December 19, 2018

Concatenate multiple datasets tool; combining all fastq.gz files under a single barcode to one fastq.gz file

Related topics