I am trying to do a transcriptome de novo assembly using Trinity but it results in errors. I tried to find here there reason for it but I couldn’t figure out what is the problem. The reads were downloded from SRA, and I runned FASTQC on it to check quality encode and it is Sanger/Illumina 1.9, so it…

Hi @andre.sa Thanks for leaving the new failed run undeleted in that same history. I see the problem (missed it the first time). The original fastq format was interleaved/interlaced. This FAQ covers some ways to convert that format to two datasets (one for forward reads, one for reverse reads): NC…

Error in de novo assembly of RNAseq using Trinity

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gbbio October 20, 2021, 2:42pm 2

If a job fails you can click that small bug icon on the red colored history “block” to see the error.

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Error in de novo assembly of RNAseq using Trinity

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