Hi all, I just noticed there is a slight difference between FASTQ files downloaded using the ‘ Faster Download and Extract Reads in FASTQ’ tool and the one published on ENA for the same accession, does anybody know why this is happening? Cheers, Rand

Welcome, @rand.zoabi Are you referring to the sequence identifiers, or the bases? Both can be a bit different, and this is why Raw reads are submitted to the SRA These are then “processed” by SRA The processed reads are what the fastq fetching tools retrieve Working with those processed reads i…