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Trinity problems? Use rnaSPAdes instead!
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14
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839
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April 13, 2024
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FreeBayes variant calling and Snpeff variant annotation
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18
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984
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Fastq unavailable -- Tool does not recognize inputs? How to check why
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9
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1267
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November 2, 2022
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Visualize with Krona
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12
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388
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August 6, 2025
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GTN Transcriptomics Hands-on: Genome-wide alternative splicing analysis. Troubleshooting reference data choices!
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27
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393
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August 19, 2025
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Troubleshooting resources for errors or unexpected results
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0
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11418
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November 28, 2018
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DESeq2 or EdgeR or Limma without replication
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8
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5162
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February 14, 2025
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How to access your Self Serve extended data storage at UseGalaxy servers!
I’m working on a transciptomics data which will probably exceed the given 250GB data. Is there any possible solution to this? for example increasing my storage?
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1
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2720
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August 12, 2024
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Scheduling recurrent workflows
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8
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308
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March 26, 2024
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How to get faster help with your question
How to get faster help with your question
We can’t help without details! :scientist: Consider Sharing your History or posting screenshots of your Job Information Details page. Confirm the Server URL (your web browser at …
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0
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1253
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January 12, 2024
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Avoid :warning: **Liftover** and try using a **CrossMap** tool instead
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2
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982
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Custom genome, SAM BAM conversion, and sort order datatypes
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3
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689
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July 20, 2023
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How to see the UseGalaxy.eu job queue statistics
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2
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794
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March 5, 2024
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Avoid :warning: **Liftover** and try using a **CrossMap** tool
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2
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770
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September 25, 2025
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MAF manipulation tools in Galaxy: step by step publication with an example shared history
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8
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771
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June 15, 2023
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DEseq2 understanding the log2 fold change
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3
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1077
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September 27, 2024
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Getting Data into Galaxy
Data Upload
Use the Upload tool to load datasets into your history
:arrow_up:
Files from your computer using Choose local files
Files with a public URL using Paste/Fetch data
Files from remote data sources using Choose…
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0
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1940
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September 29, 2023
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How to find the reference transcriptome for analysis tools: GRCh38/hg38 example
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3
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952
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May 16, 2024
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Private Galaxy Servers
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0
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1886
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September 28, 2023
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Reference genomes at public Galaxy servers: GRCh38/hg38 example
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0
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1681
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January 29, 2024
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Custom genome + custom build: How to use a genome that is not natively indexed at the server you are working at
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3
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832
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May 21, 2023
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Public Galaxy Servers
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0
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1662
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September 28, 2023
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Usage help -- single end versus paired end BAM options
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4
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737
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October 16, 2023
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Issue with StringTie Output File when using DESeq2 option
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4
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688
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May 8, 2024
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User Account Quotas, Memory and Concurrent jobs at UseGalaxy.eu
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4
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193
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April 16, 2025
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Error when running DeSeq2: help resources and input strategies
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3
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530
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August 30, 2024
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Stringtie invalid escape sequence
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2
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598
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May 16, 2024
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Length of Quality Sequence and Length of read do not match
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1
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722
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April 18, 2025
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Trinity and reads "smaller than 25 base pairs"
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1
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706
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May 3, 2022
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Missing "paired" input for fastp tool
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8
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320
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February 24, 2025
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Account problem
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1
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361
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March 18, 2024
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SPAdes error -- running out of memory/not enough memory allocated for job
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1
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637
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June 6, 2024
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Kraken2 Database Fungi
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4
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388
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October 13, 2025
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This job was terminated because it used more memory than it was allocated
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2
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410
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July 22, 2024
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FAQ: Create a Qiime2 qza artifact from a Galaxy collection in ONE STEP!
:partying_face: Try the new QZA creation method!
Creating a Qiime2 qza artifact :magic_wand: directly from a Galaxy Collection
1. Create your Galaxy Collection!
Single end will have the collection shape List
Paired end…
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0
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121
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February 25, 2026
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Job delays? How to check your job queues and how to work at multiple public UseGalaxy servers
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6
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245
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August 8, 2025
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Read normalization
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3
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264
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January 31, 2025
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ATAC Seq wig to bigwig transformation of MACS2 data
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8
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174
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August 4, 2025
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Error with HTseq RNAseq read count
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1
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369
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November 17, 2023
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Faster Download and Extract Reads in FASTQ and ENA reads are slightly different
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2
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297
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September 27, 2024
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BAM index, fasta indexes, display applications, custom genome builds, IGV
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3
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248
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September 22, 2025
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Downloading SnpEff database into history: Create database instead!
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3
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239
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July 18, 2025
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Allowlist guidence
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7
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167
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March 4, 2025
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Navigating your data storage: existing data, new data, and different storage space options with screenshots!
The program has been permanently deleted, but it still occupies 250GB of memory.How should this problem be solved?
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1
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318
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October 27, 2025
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How to analyze trimmed reads by Shovill Faster Spades Assembly tool
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2
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253
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October 31, 2024
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Assembly statistics in reference based RNA seq analysis_tools
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1
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307
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September 6, 2023
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Job execution timing and computational resources: any tool, any public Galaxy server
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4
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192
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July 18, 2024
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Correcting errors with NCBI FCS GX Foreign Contamination Screening assembled genomes
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1
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170
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July 17, 2025
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Qiime2 import troubleshooting: Retain original Illumnia file names + use simple list collection
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2
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135
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August 8, 2025
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Quality Control Start Here! multQC issue and guidance?
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1
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282
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March 3, 2025
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