Hello everyone,
I followed the ATAC-Seq tutorial to get a first insight into my ATAC Seq Data. Anyhow, when I try to convert my MACS2 bedgraph file into a bigwig file I receive this error:
bedGraph error line 24443185 of trackless: chromosome chr17 has size 81195210 but item ends at 81195213
I also found the post in this forum where someone had a similar problem, so I checked the reference genome (I used hg19) and the whole workflow up to this step, but I couldn’t find my mistake so far. I also asked KI for help, but that was also not helpful. So I try to figure out, how to get rid of those three bases, that inhibit the ongoing analysis. In the mentioned post, they were able to solve the problem, but since they didn’t share the details public, I don’t understand, which steps are required. Can someone maybe explain to me, where the problem is, and what I have to do to go on?
Many thanks in advance!