Length of Quality Sequence and Length of read do not match

[s3774248]

Hi there!
I am a student just starting out Galaxy for the first time.
I am trying to use the CutAdapt tool to trim some reads, but I get the error…
“length of quality sequence (79) and length of read (125) do not match”

any thoughts on how I can fix this?

[jennaj]

For anyone reading this later on:

Check your job details page using the i-icon. Do you see this within the message? Or a similar message about the fastq format? If yes, then your fastq data is not intact for some reason.

Sequence and quality length don’t match

Common reasons:

1. Manipulations done somewhere else impacted the file content
2. Or, a truncated file was created when moving the data between storage locations, including a potentially interrupted Upload job to Galaxy

Galaxy resources!

• To learn about NGS reads and how to inspect them, please start here: Hands-on: NGS data logistics / NGS data logistics / Introduction to Galaxy Analyses

• To learn how to Upload data to Galaxy, please see: Getting Data into Galaxy.

• To double check for intact files and the current state of compression, try the tool Fastq info at any UseGalaxy server.

• To check the quality content of fastq data, try the tool FastQC.

• To see what a simple Quality Control workflow looks like, please see → What do these FastQC results mean? - #4 by jennaj

• More workflows can be found at:

  • Training → GTN Pan-Galactic Workflow Search
  • Production/HTP → https://iwc.galaxyproject.org/
  • The Workflows → Public workflows tab at any UseGalaxy server

New to workflows? Start here: Hands-on: Galaxy Basics for everyone / Galaxy Basics for everyone / Introduction to Galaxy Analyses