Trying to do RNA seq data analysis denovo based but trinity giving error.

Please help.

Galaxy Tool Error Report

from https://usegalaxy.org/

Error Localization

Dataset 79869624 (bbd44e69cb8906b5cf64175ab8c7a2f7)

History 5619023 (07bef24c7a90ef05)

Failed Job 121: Trinity on data 56 and data 55: Gene to transcripts map (bbd44e69cb8906b57bc5840c89cd6fe5)

User Provided Information

The user *(admin redacted for privacy)* provided the following information:

None

Detailed Job Information

Job environment and execution information is available at the job info page.

Job ID 42383847 (bbd44e69cb8906b58ccb29cce2790c64)

Tool ID toolshed.g2.bx.psu.edu/repos/iuc/trinity/trinity/2.9.1+galaxy2

Tool Version 2.9.1+galaxy2

Job PID or DRM id 42383847

Job Tool Version

Job Execution and Failure Information

Command Line

if [ -z “$GALAXY_MEMORY_MB” ] ; then GALAXY_MEMORY_GB=1 ; else GALAXY_MEMORY_GB=$((GALAXY_MEMORY_MB / 1024)) ; fi ; if [ ! -z “$TRINITY_SCRATCH_DIR” ] ; then workdir=`pwd`

; scratchfolder=$(mktemp -d -p “$TRINITY_SCRATCH_DIR”); emptyfolder=$(mktemp -d -p “$TRINITY_SCRATCH_DIR”); cd “$scratchfolder” ; fi ; ln -s ‘/scratch1/03166/xcgalaxy/main/staging//42383847/inputs/dataset_6d325f88-9577-4499-9b5a-bc6044005616.dat’ left_input0.fastqsanger.gz && ln -s ‘/scratch1/03166/xcgalaxy/main/staging//42383847/inputs/dataset_641bd050-dc3a-4114-8811-acf13a33f5de.dat’ right_input0.fastqsanger.gz && Trinity --no_version_check --left ‘left_input0.fastqsanger.gz’ --right ‘right_input0.fastqsanger.gz’ --seqType fq --min_contig_length 200 --min_kmer_cov 1 --CPU ${GALAXY_SLOTS:-4} --max_memory ${GALAXY_MEMORY_GB:-1}G --bflyHeapSpaceMax ${GALAXY_MEMORY_GB:-1}G --bfly_opts ‘-V 10 --stderr’ && if [ ! -z “$TRINITY_SCRATCH_DIR” ] ; then mkdir -p “$workdir/trinity_out_dir”; cp -p trinity_out_dir/Trinity* “$workdir/trinity_out_dir”; cd “$TRINITY_SCRATCH_DIR”; rsync -a --delete “$emptyfolder/” “$scratchfolder/”; rmdir “$emptyfolder” “$scratchfolder/”; cd “$workdir”; fi ;

stderr

stdout

```
______ ____ ____ ____ ____ ______ __ __
| || \ | || \ | || || | |
| || D ) | | | _ | | | | || | |
|_| |_|| / | | | | | | | |_| |_|| ~ |
| | | \ | | | | | | | | | |___, |
| | | . \ | | | | | | | | | | |
|__| |__|\_||____||__|__||____| |__| |____/
Trinity-v2.9.1
```

Left read files: $VAR1 = [

‘left_input0.fastqsanger.gz’

];

Right read files: $VAR1 = [

‘right_input0.fastqsanger.gz’

];

Monday, May 2, 2022: 02:41:37 CMD: java -Xmx64m -XX:ParallelGCThreads=2 -jar /usr/local/opt/trinity-2.9.1/util/support_scripts/ExitTester.jar 0

Picked up _JAVA_OPTIONS: -Xmx7g -Xms256m

Monday, May 2, 2022: 02:41:38 CMD: java -Xmx4g -XX:ParallelGCThreads=2 -jar /usr/local/opt/trinity-2.9.1/util/support_scripts/ExitTester.jar 1

Picked up _JAVA_OPTIONS: -Xmx7g -Xms256m

Monday, May 2, 2022: 02:41:38 CMD: mkdir -p /scratch1/03166/xcgalaxy/main/staging/42383847/working/trinity_out_dir

Monday, May 2, 2022: 02:41:38 CMD: mkdir -p /scratch1/03166/xcgalaxy/main/staging/42383847/working/trinity_out_dir/chrysalis

## -------------- Trinity Phase 1: Clustering of RNA-Seq Reads ---------------------

##
------------ In silico Read Normalization ---------------------

– (Removing Excess Reads Beyond 200 Coverage –

# running normalization on reads: $VAR1 = [

```
[
'/scratch1/03166/xcgalaxy/main/staging/42383847/working/left_input0.fastqsanger.gz'
],
[
'/scratch1/03166/xcgalaxy/main/staging/42383847/working/right_input0.fastqsanger.gz'
]
];
```

##
Monday, May 2, 2022: 02:41:38 CMD: /usr/local/opt/trinity-2.9.1/util/insilico_read_normalization.pl --seqType fq --JM 190G --max_cov 200 --min_cov 1 --CPU 56 --output /scratch1/03166/xcgalaxy/main/staging/42383847/working/trinity_out_dir/insilico_read_normalization --max_CV 10000 --left /scratch1/03166/xcgalaxy/main/staging/42383847/working/left_input0.fastqsanger.gz --right /scratch1/03166/xcgalaxy/main/staging/42383847/working/right_input0.fastqsanger.gz --pairs_together --PARALLEL_STATS

-prepping seqs

Converting input files. (both directions in parallel)CMD: seqtk-trinity seq -A -R 1 <(gunzip -c /scratch1/03166/xcgalaxy/main/staging/42383847/working/left_input0.fastqsanger.gz) >> left.fa

CMD: seqtk-trinity seq -A -R 2 <(gunzip -c /scratch1/03166/xcgalaxy/main/staging/42383847/working/right_input0.fastqsanger.gz) >> right.fa

CMD finished (73 seconds)

CMD finished (74 seconds)

CMD: touch left.fa.ok

CMD finished (0 seconds)

CMD: touch right.fa.ok

CMD finished (0 seconds)

Done converting input files.CMD: cat left.fa right.fa > both.fa

CMD finished (11 seconds)

CMD: touch both.fa.ok

CMD finished (0 seconds)

-kmer counting.

##
----------- Jellyfish --------------------

– (building a k-mer catalog from reads) –

CMD: jellyfish count -t 56 -m 25 -s 27321675151 --canonical both.fa

CMD finished (95 seconds)

CMD: jellyfish histo -t 56 -o jellyfish.K25.min2.kmers.fa.histo mer_counts.jf

CMD finished (11 seconds)

CMD: jellyfish dump -L 2 mer_counts.jf > jellyfish.K25.min2.kmers.fa

CMD finished (31 seconds)

CMD: touch jellyfish.K25.min2.kmers.fa.success

CMD finished (0 seconds)

-generating stats files

CMD: /usr/local/opt/trinity-2.9.1/util/…//Inchworm/bin/fastaToKmerCoverageStats --reads left.fa --kmers jellyfish.K25.min2.kmers.fa --kmer_size 25 --num_threads 28 --DS > left.fa.K25.stats

CMD: /usr/local/opt/trinity-2.9.1/util/…//Inchworm/bin/fastaToKmerCoverageStats --reads right.fa --kmers jellyfish.K25.min2.kmers.fa --kmer_size 25 --num_threads 28 --DS > right.fa.K25.stats

-reading Kmer occurrences…

-reading Kmer occurrences…

done parsing 81736069 Kmers, 81736069 added, taking 87 seconds.

Sequence: CCGTCTTGGACCAAACTAis smaller than 25 base pairs, skipping

Sequence: TGATGATGTASequence: GGCAAAAis smaller than 25 base pairs, skippingis smaller than 25 base pairs, skipping

Sequence:

Sequence: Sequence: Sequence: CTAGTGCCACAGCACAGAGCCCACGGSequence: AGGTGCATCAGTis smaller than 25 base pairs, skipping

is smaller than is smaller than 25 base pairs, skipping25CGTGTTCTTGCTGAAGis smaller than AGGAGCCTGSequence: CTCTAGCACis smaller than 25 base pairs, skipping

base pairs, skippingSequence: CTTAGGis smaller than

25 base pairs, skipping

Sequence: 25Sequence: TTTCAAAATCAGTAGGCAAAAis smaller than base pairs, skipping

CGGCAAG25is smaller than is smaller than base pairs, skippingSequence: CGGGGATGGATCAACACAAGCCis smaller than 25 base pairs, skipping2525

base pairs, skipping base pairs, skipping

Sequence: ATGATGACTGTCGAAAis smaller than 25 base pairs, skipping

Sequence: GTCCis smaller than 25 base pairs, skippingSequence:

TAAGAAGGGTAis smaller than 25 base pairs, skipping

Sequence: TTCAAGGAAAGTGAGTCGis smaller than 25 base pairs, skippingSequence:

Sequence: GGCCACATCSequence: GTTTCATGGCTCAAis smaller than Sequence: is smaller than 25is smaller than CATCCCis smaller than base pairs, skipping

2525 base pairs, skipping base pairs, skipping25

Sequence: Sequence: CCACTTAGis smaller than 25 base pairs, skipping

base pairs, skipping

Sequence: CTCCATTGCTCis smaller than 25 base pairs, skippingSequence: ACAAAis smaller than CTTGTTTCATGis smaller than 25 base pairs, skipping

Sequence: CCTGACTis smaller than 25Sequence: Sequence: 25 base pairs, skippingCCCGCGTCTAGTCGATTCCTTCis smaller than 25

Sequence: CAGCAGis smaller than 25 base pairs, skipping

base pairs, skipping

Sequence: GTCCGAAGGGCAGTSequence: CTAGGACis smaller than 25 base pairs, skipping

base pairs, skippingis smaller than 25 base pairs, skippingGGCATTGGCACTGTGCis smaller than 25

base pairs, skippingSequence:

Sequence: CCCCGATCCAGTTCTCGCATCTGTis smaller than GCACT25is smaller than base pairs, skipping25

base pairs, skippingSequence:

CTGTCis smaller than 25 base pairs, skippingSequence:

TTCAAATCTCAAAis smaller than 25 base pairs, skipping

Sequence: GCCCTCACAACCAis smaller than 25 base pairs, skipping

Sequence: CCGCTis smaller than 25 base pairs, skippingSequence: Sequence:

ATTGAAAGGAAATACAAATCTGATis smaller than 25is smaller than Sequence: CTGTTCG base pairs, skipping

Sequence: is smaller than 25 base pairs, skipping

Sequence: AGACCACCGTCTCCGAis smaller than 25 base pairs, skipping

Sequence: AACAGAAACAAGTAAACis smaller than 25 base pairs, skippingSequence: ACGGGGAAGAATAis smaller than 25 base pairs, skipping

25 base pairs, skipping

CAGGTGACATGGTTis smaller than

Sequence: 25Sequence: GTGGCGC base pairs, skippingCAAGCATCCGAGGACis smaller than

is smaller than 2525 base pairs, skipping base pairs, skipping

Sequence:

Sequence: CTGATCACCACGCTGTACTCGTACis smaller than 25Sequence: CTCAACTis smaller than 25 base pairs, skipping

Sequence: CGTATis smaller than 25 base pairs, skippingSequence: ATAAACTTTACCCTGCis smaller than 25 base pairs, skipping

is smaller than Sequence: ATGAGATGTis smaller than 25 base pairs, skipping

Sequence: AAGGAATACGAATCTCAis smaller than 25 base pairs, skipping

Sequence: CGCGGTGis smaller than 25Sequence: CCTTAACATTACTTAATACCis smaller than 25 base pairs, skipping

base pairs, skipping

base pairs, skipping25

base pairs, skipping

Sequence: Sequence: Sequence: Sequence: TGTAATTGCTTTCACCAACAACAACGTAis smaller than 25is smaller than TGAAGGCTCTTGis smaller than base pairs, skipping25 base pairs, skipping

Sequence:

is smaller than 25GGCTGTCGAAGAGTCGTT25Sequence: base pairs, skipping

base pairs, skippingGGCCAAis smaller than Sequence: CTCCCCCTGAATGAGis smaller than 25 base pairs, skippingis smaller than 25 base pairs, skipping

25Sequence:

base pairs, skippingSequence: CGTTACACATGTis smaller than 25GGCGSequence: CCGGACGis smaller than base pairs, skipping

Sequence:

25is smaller than Sequence: CCAASequence: CACCis smaller than 25 base pairs, skipping

base pairs, skipping

CCTCAACACTTGCAGCis smaller than 25 base pairs, skippingis smaller than 25Sequence: CGGCGCGGGCAGAGGis smaller than 25 base pairs, skippingSequence: GCTACis smaller than 25 base pairs, skipping

25 base pairs, skipping

Sequence: ACCGAis smaller than 25

Sequence: AGGGC base pairs, skipping

is smaller than base pairs, skipping25 base pairs, skippingSequence: Sequence: CAGGCAGTGGTAGAGAAis smaller than 25 base pairs, skipping

Sequence: Sequence: Sequence: CGAGAGGGAGACAAis smaller than 25 base pairs, skipping

CTACCTAATCAAis smaller than Sequence: TGTTGAGis smaller than 2525 base pairs, skipping

GCCCGTTAGCCCTCTCTGCAA base pairs, skipping

Sequence: is smaller than 25 base pairs, skippingSequence: CCGACAAATCCATis smaller than 25 base pairs, skippingCCGGGTGTTTGACis smaller than 25

CGCSequence: base pairs, skippingis smaller than CCTGCCATCATASequence: TGGAGACGGCAACGAGGTGG25 base pairs, skipping

is smaller than Sequence: is smaller than 25 base pairs, skipping25GGGGAAGTAACGCTGGTis smaller than Sequence: base pairs, skipping

25Sequence: CCCCis smaller than base pairs, skippingCTCGGGTGGCGCACTTCTTGGGis smaller than 25

25 base pairs, skipping

Sequence: Sequence: Sequence: GGCTGCCGCAAGGACCATCG base pairs, skippingAATCACTTGAGis smaller than 25

is smaller than 25CACGTCSequence: base pairs, skippingSequence: GCCGGTGCGGCCGATGCis smaller than 25 base pairs, skippingSequence: CGTTCCTTCACGTAis smaller than 25 base pairs, skipping

base pairs, skippingCCCCTCCTC

Sequence: GCTACCCTTCTTCTTCis smaller than 25 base pairs, skipping

is smaller than 25 base pairs, skipping

Sequence: CCCGCCGTCis smaller than 25 base pairs, skipping

Sequence: CTCTGCCGCAGTAGGTCTGACis smaller than 25 base pairs, skipping

Sequence: CCCACACGAACCACACCCTis smaller than 25 base pairs, skipping

Sequence: Sequence: CGCCAGATCACCGCCGAGGASequence: CGCCAAGAis smaller than 25Sequence: CCTGGATGTis smaller than 25 base pairs, skipping

GCCis smaller than Sequence: GGACTATis smaller than 25 base pairs, skipping

Sequence: CGAis smaller than 25 base pairs, skipping

is smaller than 25 base pairs, skipping

Sequence: Sequence: ATCAATAAACATGTGis smaller than is smaller than 25 base pairs, skipping

25CTCCAGCCGis smaller than 25Sequence: GATCTCis smaller than 25Sequence: CTCGGAis smaller than 25 base pairs, skipping

base pairs, skipping25 base pairs, skipping

base pairs, skipping

base pairs, skipping

base pairs, skipping

Sequence: CCTGCGis smaller than 25Sequence: CGTGGTGGTGCTCGCCATCCTTCTis smaller than Sequence: Sequence: CGACAATATCGACAC25AGACCTTAGATTTTCATCCGis smaller than 25 base pairs, skipping

base pairs, skipping base pairs, skippingis smaller than

Sequence:

25Sequence: AGAAGATAAGCGAis smaller than TCGTCCCCTTTis smaller than base pairs, skipping25 base pairs, skipping25 base pairs, skippingSequence: GAAGTCGAAGGCCis smaller than 25 base pairs, skipping

Sequence:

ATTTTCTCis smaller than 25Sequence: base pairs, skippingCTTCCTTGAGCTG

Sequence: is smaller than 25CACCTGCGCAGCCCTT base pairs, skippingSequence: is smaller than 25 base pairs, skippingCCGATTCTCGTTGCTCAAGGGTSequence: CCCGTAATACis smaller than 25

Sequence: base pairs, skipping

Sequence: CTACTGis smaller than Sequence: TGGCCTTATTGCTGCAis smaller than 25CAACis smaller than 25 base pairs, skipping

25 base pairs, skippingSequence: CGGGGAGTAAGCCGGAis smaller than

is smaller than base pairs, skipping

25 base pairs, skipping

25 base pairs, skipping

Sequence: Sequence: CTGis smaller than Sequence: GGGCGGGCAGACGCC25Sequence: GGAGis smaller than Sequence: CGTGGATGTGTCis smaller than 25 base pairs, skipping

Sequence: CTGTTGGTTCTGCTis smaller than 25 base pairs, skipping

is smaller than 25 base pairs, skipping

base pairs, skipping

Sequence: Sequence: ATCCCATCCAATGAGAAATCAis smaller than CAAGSequence: AGATAAGAGTGGCGATGGCTis smaller than 25 base pairs, skipping

CCTGC25 base pairs, skipping

is smaller than is smaller than 25Sequence: TGTis smaller than 2525 base pairs, skipping base pairs, skipping base pairs, skipping

25 base pairs, skippingSequence: Sequence: CTGCAGGTTCTTCTCGACAAis smaller than Sequence: CCTTis smaller than

25AAGAGGGCGTCCAAGASequence: CGACCATATCCGTC base pairs, skippingis smaller than 25is smaller than 25 base pairs, skipping

Sequence:

25 base pairs, skippingSequence: GCCSequence: AAGGCATAACCCCTTis smaller than 25is smaller than 25

CACCG base pairs, skipping

base pairs, skippingis smaller than base pairs, skipping

Sequence: TTCGTGTCGG25 base pairs, skipping

is smaller than Sequence:

Sequence: CCATTGis smaller than 25ATTTTis smaller than 25 base pairs, skipping

25Sequence: CCTCSequence: CTTGCis smaller than 25 base pairs, skipping

base pairs, skipping

base pairs, skippingis smaller than

Sequence: 25 base pairs, skipping

Sequence: Sequence: AGCGGAGCTTGTTis smaller than 25Sequence: GCCTGGGTSequence: CATTAATCCCTTAAAGis smaller than 25 base pairs, skipping

CCAAGAAGis smaller than 25 base pairs, skipping

Sequence: Sequence: ATAAAACGCGGATCis smaller than base pairs, skipping

ACCG25 base pairs, skipping

Sequence: CGACis smaller than 25 base pairs, skipping

is smaller than 25 base pairs, skipping

is smaller than GTCACGATAACATACTGGAATCTis smaller than 25 base pairs, skipping

Sequence: CGACTGGAGATCis smaller than 25 base pairs, skipping

Sequence: Sequence: AAGGCATAACCCCTTATGACis smaller than 25 base pairs, skipping

GGT25Sequence: CTCCGGAGis smaller than Sequence: CTTGCGGCAGCCT base pairs, skipping

Sequence: ACTGAis smaller than 25Sequence: TATTGCTCTis smaller than 25 base pairs, skipping

is smaller than is smaller than 25 base pairs, skipping

2525Sequence: base pairs, skipping

GTTCATCCTCC base pairs, skipping base pairs, skippingis smaller than

Sequence:

25CAGACCG base pairs, skippingSequence: ACAAGATCACACAGCTCACGGATAis smaller than is smaller than

2525Sequence: TCGGTAGGCTCTCGCTGC base pairs, skipping base pairs, skippingSequence: ATAGAACCGis smaller than

is smaller than

25Sequence: 25 base pairs, skippingCTCCATTCTCAGTCCCGGCAA base pairs, skipping

is smaller than Sequence: CCTTGAis smaller than 25 base pairs, skipping

25Sequence: GGAA base pairs, skipping

is smaller than Sequence: CGCCATSequence: CAAAGCGCTACCis smaller than 25 base pairs, skipping

25 base pairs, skippingis smaller than

25 base pairs, skipping

Sequence: CGCTGGACTGGTis smaller than Sequence: CGTAGCTCCC25is smaller than 25 base pairs, skippingSequence: base pairs, skipping

CCGAAACCCTAACCSequence: TCTCTTCTC

is smaller than 25is smaller than 25 base pairs, skipping

Sequence: CTAGGGCAGATTCTis smaller than 25 base pairs, skipping

Sequence: CTCCAis smaller than 25Sequence: GTTCGis smaller than 25 base pairs, skipping

Sequence: CCTCGCCCACTTis smaller than 25 base pairs, skipping

Sequence: CTGTTGis smaller than 25 base pairs, skipping

base pairs, skipping base pairs, skipping

Sequence: CTCCCCACGGAGACTTTCTTGis smaller than 25 base pairs, skipping

Sequence: TGCTCTTCCCAAGTTCCGCAAGCTis smaller than 25 base pairs, skipping

Sequence: TGGCACGGis smaller than Sequence: 25CGACCGSequence: base pairs, skippingSequence: CGATCCTCGAis smaller than 25 base pairs, skipping

is smaller than ACSequence: CTTCGAGCTTCTCATCCCGCAis smaller than 25 base pairs, skipping

25 base pairs, skipping

is smaller than

Sequence: 25CTCAis smaller than 25 base pairs, skipping base pairs, skipping

Sequence: GTCAACGis smaller than Sequence: 25Sequence: AAGGATCTCACCAGCATG base pairs, skippingTAGAis smaller than Sequence:

is smaller than 25ATGTCTACAATCTATGATGAis smaller than 25 base pairs, skipping base pairs, skipping

Sequence: 25 base pairs, skipping

ATGAAGCTG

Sequence: is smaller than GTTACGGT25is smaller than base pairs, skipping25

Sequence: base pairs, skippingGTAGGAGTT

is smaller than 25 base pairs, skippingSequence: Sequence: Sequence: CTGGTCAAACTATACTGAACTCCGATTTATCCTTAis smaller than 25is smaller than

is smaller than base pairs, skipping25 base pairs, skipping

25

Sequence: base pairs, skippingCCCAGCTGAAACTAACAAACis smaller than

25 base pairs, skipping

Sequence: TTGTCATCAGCTTTCAGTGATAGis smaller than 25 base pairs, skipping

Sequence: Sequence: AGACAGGCCGTTGGATTGCCCSequence: is smaller than is smaller than Sequence: CTCGATGGATCAAAGAAAGCCATTis

…

than 25 base pairs, skipping

Sequence: is smaller than 25GCTGGGTGSequence: base pairs, skippingSequence: ATACGGTGTis smaller than 25 base pairs, skipping

GTGGA

is smaller than Sequence: Sequence: GGAAGATGG25 base pairs, skipping

is smaller than 25 base pairs, skippingis smaller than 25 base pairs, skipping

Sequence: Sequence: GTTGATGTTGCTGAAGCCAAis smaller than 25CAAAGSequence: GGAAGACACis smaller than 25TGATG base pairs, skipping

base pairs, skippingis smaller than 25

base pairs, skipping

is smaller than Sequence: 25 base pairs, skippingGGCCCAAACAT

is smaller than 25 base pairs, skipping

Sequence: TGGAAGAGAASequence: is smaller than Sequence: TCCTTis smaller than ATCTAACCCA2525is smaller than base pairs, skipping base pairs, skipping25

base pairs, skippingSequence:

CTTCGAAACGGATCAis smaller than Sequence: 25Sequence: CGGAAis smaller than 25 base pairs, skipping

Sequence: Sequence: GTTCATACAis smaller than GACACGAATATGTACGGAACCTis smaller than 25 base pairs, skipping

base pairs, skipping

25CAGGAAASequence: Sequence: CTTGCCCCTTis smaller than ATCGCC base pairs, skipping

25 base pairs, skipping

Sequence: Sequence: is smaller than 25is smaller than 25 base pairs, skipping

Sequence: GTTTis smaller than 25 base pairs, skipping

TTCCTis smaller than 25 base pairs, skipping

base pairs, skipping

Sequence: CACCACSequence: CCCAGis smaller than 25 base pairs, skipping

CTAAACTTCAATCTis smaller than Sequence: Sequence: is smaller than 25CTATAAATGATTTGGCTis smaller than 25 base pairs, skipping

25Sequence: base pairs, skippingSequence: CGCCGis smaller than 25 base pairs, skipping

CCACCATCAACAGCAATTGTCis smaller than base pairs, skipping

GAAGAGGG25

is smaller than base pairs, skipping

25Sequence: CAAAGGTATGAGAAGAGGis smaller than base pairs, skipping25

base pairs, skipping

Sequence: Sequence: GTGTASequence: CGGCis smaller than 25GCCAAATCGAATCGAGGAGACTTTis smaller than 25 base pairs, skippingSequence: CAAGGTTGis smaller than 25 base pairs, skipping

Sequence: GTTCAAACTTGATAAis smaller than base pairs, skipping

Sequence: CTAGAAis smaller than 25

is smaller than 25 base pairs, skipping25 base pairs, skipping

base pairs, skipping

Sequence:

CGTGCAGTAGCTGTGis smaller than 25 base pairs, skipping

Sequence: GGAGGAis smaller than Sequence: 25 base pairs, skipping

Sequence: Sequence: Sequence: CCCACAAACTAATAAis smaller than 25 base pairs, skipping

CTGAAGATGGTCTCGGTCAGAGTATis smaller than is smaller than 25 base pairs, skipping25CAAGGCGGTSequence: CGCAGGTGTCCTAAGATis smaller than 25is smaller than 25 base pairs, skipping

Sequence: base pairs, skipping

CTCCTTTCACGCCTTGTCTTTTAT base pairs, skippingSequence: is smaller than 25 base pairs, skipping

CCGTGCATATACTACTGCCTCTSequence: is smaller than GGCGAGGGTTCAis smaller than 2525 base pairs, skipping

Sequence: GCAACGTCis smaller than base pairs, skippingSequence: ACGACTAGCTGCis smaller than

Sequence: AACTC25Sequence: Sequence: CCAATCAis smaller than 25 base pairs, skipping

base pairs, skipping

is smaller than GCTCis smaller than 2525 base pairs, skipping base pairs, skipping

Sequence: AATCTCACAAACACATTATTCis smaller than 25 base pairs, skipping

25Sequence: Sequence: CTCCACATGCTGATGAAACCSequence: GGCGGCCCGCAAis smaller than 25is smaller than 25 base pairs, skipping

Sequence: ATGCAAATGCis smaller than 25 base pairs, skipping

base pairs, skippingSequence: CTAGTGTCGTCGTTCCTCCTCGis smaller than 25

CGGCATAGTGGis smaller than 25 base pairs, skipping

base pairs, skipping

base pairs, skippingSequence: CCTGTGGAAGCCCCTCCSequence: TGAAGis smaller than 25 base pairs, skipping

Sequence: GGCCGTTGGAGis smaller than

is smaller than 2525 base pairs, skippingSequence: base pairs, skipping

TTGTAAGTGGCTCGTAC

is smaller than 25 base pairs, skippingSequence: CCGCAAGCACCAis smaller than Sequence: GTTTGGAis smaller than 25 base pairs, skipping

25Sequence: Sequence: CCCAAATTGAGTACTCGGGAAC base pairs, skipping

Sequence: GTCTCCGGTAACGCTCTCAAis smaller than 25 base pairs, skippingis smaller than AGTTAAGACT25 base pairs, skipping

Sequence: CTCGGAis smaller than 25 base pairs, skipping

Sequence:

Sequence: is smaller than 25GACCGis smaller than 25 base pairs, skipping base pairs, skipping

CTGATATCCGis smaller than 25 base pairs, skipping

Sequence: CGTAAT

Sequence: is smaller than Sequence: GGACis smaller than 25 base pairs, skipping

25TGTTT base pairs, skippingis smaller than

25Sequence: CGCCCTTGACCis smaller than Sequence: CTGTGATGAGTGTGGTATCis smaller than 25Sequence: ATCTCis smaller than 25 base pairs, skipping

Sequence: CTTCGCCGCCATCTCGCTCTGGis smaller than 25Sequence: GTACAAGCTGCTATACT base pairs, skipping

base pairs, skipping25 base pairs, skipping base pairs, skipping

is smaller than

Sequence:

25TCTACATTATAGTAA base pairs, skipping

is smaller than 25 base pairs, skipping

Sequence: Sequence: Sequence: GTTGTACGAAATACGis smaller than is smaller than is smaller than 252525 base pairs, skipping base pairs, skipping base pairs, skipping

Sequence:

Sequence: CCACGSequence: CAGGCGAAGGTGCCis smaller than TAAAAACTTCAis smaller than 25 base pairs, skipping

Sequence: Sequence: CTCCTCAGCACCCTCCTTTis smaller than 25 base pairs, skipping

Sequence: Sequence: 25 base pairs, skipping

Sequence: CCTAis smaller than 25CTAGTTTACAACTis smaller than 25 base pairs, skipping

Sequence: CCGCTis smaller than 25 base pairs, skipping

CGCTis smaller than 25 base pairs, skippingis smaller than base pairs, skipping

25Sequence: GTACCGCGTTCACTGTGis smaller than 25CAAGAATGTTGCTGTCAAGGAis smaller than base pairs, skipping

25 base pairs, skipping base pairs, skipping

Sequence: CGAGCGATCACCGis smaller than 25 base pairs, skipping

Sequence: CTCCTTCATTCTCCTTGGCTTCCis smaller than 25 base pairs, skippingSequence: CCTCGTis smaller than Sequence: CCAACAATTAis smaller than 25 base pairs, skipping

25Sequence: base pairs, skippingSequence: AGCAGTTGCTTCAis smaller than GGACA

Sequence: Sequence: AAGAAAGGis smaller than 25 base pairs, skipping

25CTGAis smaller than base pairs, skippingis smaller than 25 base pairs, skipping25

Sequence: base pairs, skipping

Sequence: AAAGGCTAis smaller than 25CTCAGTGCACGCCGCCAis smaller than 25 base pairs, skipping

Sequence: Sequence: GCAAAGCTCGATTTGGGSequence: base pairs, skipping

is smaller than Sequence: Sequence: TCCAGATACAAATTACTTATTTAis smaller than 25 base pairs, skipping

Sequence: CAGGGis smaller than 25 base pairs, skipping

CAGTTCTCCGTGTTTCTGCAAis smaller than 25 base pairs, skipping

CCTAGG25 base pairs, skippingCTGCTGis smaller than 25Sequence: CTGCis smaller than 25 base pairs, skipping

Sequence: is smaller than 25 base pairs, skipping

CTCGAGGTTAGGGTSequence: base pairs, skippingSequence: GCCTCTTCCCCCis smaller than 25 base pairs, skipping

CGACCAGAGGAis smaller than 25 base pairs, skipping

is smaller than 25

Sequence:

base pairs, skippingCGCGGACTGAATCTSequence: AACCGACTGTGis smaller than 25 base pairs, skipping

is smaller than

Sequence: 25Sequence: base pairs, skipping

CCCCGAACGCCAATCCCAGTGATTCTCTGGis smaller than is smaller than 25 base pairs, skipping25 base pairs, skipping

Sequence:

Sequence: GTGGSequence: GGCTTGGAis smaller than 25is smaller than GCAGGTTTCCGTTTTTTCis smaller than 25Sequence: base pairs, skipping

base pairs, skippingACACCis smaller than 25Sequence: 25

base pairs, skippingCAGAAACATGCCTGACis smaller than 25 base pairs, skipping

base pairs, skippingSequence:

GTTASequence: is smaller than CTCTis smaller than 25 base pairs, skipping

25 base pairs, skipping

Sequence: TGAGis smaller than 25 base pairs, skipping

Sequence: CGAAGCCAAAGACTGTTCCCAAis smaller than 25 base pairs, skipping

Sequence: CTGAATCTis smaller than 25Sequence: base pairs, skippingCTTTGGA

is smaller than 25 base pairs, skipping

Sequence: CTCATGGAACATTTACCis smaller than 25 base pairs, skipping

Sequence: CAGTACCis smaller than 25 base pairs, skippingSequence:

Sequence: CGGAGAGGGCGGGGGGGAAAGSequence: CTGAGis smaller than Sequence: CCCTGATGGTTACCTGTTTGAGis smaller than 25 base pairs, skippingis smaller than 25is smaller than 2525 base pairs, skipping

base pairs, skippingSequence:

base pairs, skipping

TGTGATATATATGTCCCTTGATCASequence: is smaller than GCCCCTTATCTCGCTCTTTCTG25is smaller than base pairs, skipping25Sequence: base pairs, skipping

Sequence: CATGAATACTAGTACis smaller than 25Sequence:

CCAA base pairs, skippingTGGGACTCCAACGCis smaller than

Sequence: is smaller than 25GGC25 base pairs, skippingis smaller than base pairs, skipping

25Sequence:

base pairs, skippingGGATGATCAGTAC

is smaller than 25 base pairs, skipping

Sequence: GTCGACGCCGAAis smaller than Sequence: 25Sequence: ATCACATTCTCCAAis smaller than Sequence: CGAAGAGTGTATTTGAAGTGCis smaller than 25 base pairs, skipping

base pairs, skippingSequence: GCTATACTGCCCCAGTGTCCGACGTCTCCTGCC

25Sequence: CTCGGAAis smaller than base pairs, skipping

is smaller than is smaller than 25 base pairs, skippingSequence: 25 base pairs, skipping

CAACTTT25Sequence: CACAACATis smaller than base pairs, skippingis smaller than Sequence: GGGAAATis smaller than 25 base pairs, skipping

Sequence:

25Sequence: 25ATTGCCTGGCGCCis smaller than 25 base pairs, skippingSequence: CACCTCis smaller than base pairs, skipping

base pairs, skippingGGATATTGGAATGGTis smaller than 25 base pairs, skipping

Sequence: GGAAGis smaller than 25 base pairs, skipping

25

Sequence:

Sequence: base pairs, skippingATCCCis smaller than 25Sequence: GGAGGCTis smaller than base pairs, skipping

AACGA

25Sequence: GTTGCis smaller than is smaller than base pairs, skipping25 base pairs, skipping

25

Sequence: CCGTAGCGGTGCCTACATTGSequence: CGAACACTTCis smaller than 25is smaller than 25 base pairs, skippingSequence: CTGAACCTTATAis smaller than 25 base pairs, skipping

base pairs, skipping

base pairs, skipping

Sequence: Sequence: Sequence:

CGCCCTTGACCCGGTATis smaller than is smaller than 25 base pairs, skipping25GAAGAATSequence: AGCCGTTCGGGGTT base pairs, skippingis smaller than 25is smaller than 25 base pairs, skipping

base pairs, skippingSequence: CCGCAGATATCACAAGis smaller than Sequence: CGAGGTCAGTGCAGTis smaller than 25 base pairs, skipping

Sequence: GCCTAATACGGCCGACACis smaller than 25 base pairs, skipping

25Sequence: CGGGGis smaller than 25 base pairs, skipping

Sequence: GGCGCACACATCGATAAAACCCA base pairs, skippingis smaller than

25Sequence: CCGAAis smaller than base pairs, skipping

Sequence: Sequence: GCTGTGGGTCTGCCTGCTATGis smaller than 25 base pairs, skipping

is smaller than Sequence: AGCTCGGGAAis smaller than 25Sequence: 25 base pairs, skipping

CATTCCTGAis smaller than base pairs, skippingSequence: 25 base pairs, skipping

Sequence: GAAGAAGis smaller than 25CTACATGCTCGGCAGCCis smaller than 25 base pairs, skipping25 base pairs, skipping

base pairs, skipping

Sequence: GTGGTCTis smaller than Sequence: Sequence: 25CAGGGTCGGAGACAAATCCAATGTis smaller than is smaller than 25Sequence: CTCAGTCTTGACCTGCGis smaller than 25Sequence: CCACATATis smaller than base pairs, skipping base pairs, skipping

base pairs, skipping25

25 base pairs, skipping base pairs, skipping

Sequence: Sequence: Sequence: GTTCGACCAAAAAAGCCTGAGCCCGGCGACGAGAGCCGGCTGTTACGACGGCis smaller than is smaller than is smaller than 2525 base pairs, skipping

25 base pairs, skipping

Sequence: CATTAAGAis smaller than Sequence: base pairs, skipping25CCACCTTAAATCCTCAAATTis smaller than Sequence: base pairs, skipping

25GCATCTATTGCTGATTCATis smaller than 25

base pairs, skipping base pairs, skippingSequence:

CCCTGTCATCTAGTGTCGGTTGATSequence: TGGGis smaller than 25is smaller than 25 base pairs, skipping base pairs, skippingSequence: CCAATAis smaller than

Sequence: CCGGCCAAGCAAGTis smaller than Sequence: 25 base pairs, skipping

CCGATATCTCAATTACAGCTTCCTSequence: 25is smaller than 25 base pairs, skippingGAGG base pairs, skipping

is smaller than

25Sequence: base pairs, skippingCGGGATTCCTCA

Sequence: is smaller than TGAATGCTTACT25is smaller than Sequence: CCTCCCCCATCGAGTTATCAACis smaller than base pairs, skipping25

25 base pairs, skippingSequence: TGTAAATCTTCCGACTGSequence: ATTCis smaller than

base pairs, skippingis smaller than 25 base pairs, skipping

25 base pairs, skippingSequence: ACGCCCCCGGCGCAGCCGCis smaller than 25 base pairs, skipping

Sequence: CTGCCTGCAAGTGTGGCAGTGis smaller than

Sequence: Sequence: Sequence: 25 base pairs, skippingGTCCTCGCTCTGTCCGCCGCCATTTis smaller than 25 base pairs, skippingSequence: ATCTCis smaller than 25

GCCis smaller than 25 base pairs, skippingSequence: CCACAACCCACCGTGCCCCACTCis smaller than 25