Welcome @bahar125d
Let’s start over in a new topic to review your results.
The bulk of your reads are either not mapping uniquely to a feature, or are not matching to the same coordinates as any feature at all!
The first place I usually start is with examining the reference data choices. Which genome are you using? Where did you source the feature annotation reference? Have you confirmed that both are based on the same genome assembly?
This topic explains a bit more for human, but any species can have multiple assemblies, so the advice here, especially the review and troubleshooting advice, is where to start.
Then many of the topics with these tags are discussing a similar situation featurecounts reference-annotation
Please give that a try and let us know what happens! If you need help with the review, you are welcome to share back your history in your reply and we can try to advise! ![]()