Heatmaps of DEGs presented as log2(FC)/RNA-seq

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Hello,

A slightly different question (compare to a previous post of mine), which potentially leads to a different visualization of my DEGs:

Again the start is the same:

I have used DESeq2 to identify DEGs. What I would like to do next, is to generate a heatmap (via the Heatmap2 tool), where I should show those DEGs (cut off: p<00,5 & -1<Log2(FC)>1), presented as log2(FC).

Question:

By choosing:

  1. As input for the Heatmap2 tool the rLog2 Normalized counts file that DESeq2 gave me.

  2. Choosing the option Log2(value) transform my data in the Data transformation parameter of the Heatmap2 tool.

Do I get then a heatmap presenting my DEGs, where its color key corresponds to Log2FC? If not, how can I do that (input, tools, process)

Cheers,

Manolis

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