I’ve been using IGV to visualize my BAM files, but I need to calculate the FPKM values by hand. Counting the number of reads by hand is almost impossible and tedious to do. Is it possible to quantify reads in a BAM file?
I’ve been using IGV to visualize my BAM files, but I need to calculate the FPKM values by hand. Counting the number of reads by hand is almost impossible and tedious to do. Is it possible to quantify reads in a BAM file?