HUMAnN error version

[Reki]

Galaxy Microbiology

HUMAnN 3.9+galaxy1

Error:

"The MetaPhlAn taxonomic profile provided was not generated with the

database version v3 or vJun23"

The only MetaPhlAn marker database available under

“Locally cached” is:

mpa_vOct22_CHOCOPhlAnSGB_202212

The vJun23 database required by HUMAnN does not appear

in the dropdown menu.

How can HUMAnN be run on microbiology.usegalaxy.eu using the

required vJun23 database?

[jennaj]

Welcome @Reki

Please have a look at the protocol here to see if also helps with your error? Please come back after with follow up questions, or to let us know if it worked!

@jennaj

To incorporate HUMAnN with MetaPhlan, please try following the protocol outlined in this tutorial and associated workflows!

• Hands-on: Metatranscriptomics analysis using microbiome RNA-seq data / Metatranscriptomics analysis using microbiome RNA-seq data / Microbiome

The reads are prepared, then sent through both tool pipelines independently, then the results from each are merged together after. This is what allows the reference data sources to work together.