HUMAnN fails due to MetaPhlAn database mismatch on usegalaxy.eu

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1

Hello Galaxy team,

I am trying to run HUMAnN (v3.7 / v3.9) on usegalaxy.eu for human fecal shotgun metagenomic reads.- just 2 samples , I converted fastq to fasta as well even then the error continued as I looked deeper, all HUMAnN runs fail immediately with the following error:
ERROR: The MetaPhlAn taxonomic profile provided was not generated with the database version v3 or vJun23 . Please update your version of MetaPhlAn to at least v3.0 or if you are using MetaPhlAn v4 please use the database vJun23.

In the HUMAnN tool form - “Database with clade-specific marker genes”, the only available MetaPhlAn database is:

  • mpa_vOct22_CHOCOPhlAnSGB_202212
CRITICAL ERROR: The directory provided for ChocoPhlAn contains files ( g__Bacteroides.s__Bacteroides_vulgatus.centroids.v296_201901b.ffn.gz ) that are not of the expected version. Please install the latest version of the database: v201901_v31

Is there an updated Metaphlan and chocophlan version in galaxy.eu platform that I can use to re- run HuMann?
Thanks for your attention and looking into this matter !

Best ,
Sri

Screenshot (49)
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2

Hello @sri_microbiome

To incorporate HUMAnN with MetaPhlan, please try following the protocol outlined in this tutorial and associated workflows!

The reads are prepared, then sent through both tool pipelines independently, then the results from each are merged together after. This is what allows the reference data sources to work together.

Hope this helps! :slight_smile: