Hi there! I am a baby researcher, a post-bac on my second microbiome analysis project. I am on the second step and I am already stuck. On my previous project, I was working with Illumina data, so I received raw data as unpaired sets and was, therefore, able to follow the tutorial exactly. It went ve…

Hello @n_griffith42 These are PacBio long reads, correct? What are your goals? Assemble the reads? Call SNPs? Assign taxonomy/function?

The samples are bayou water sample from different time points. I am one of the only people in my lab group that has done any sequencing analysis before. The PIs want me to do what I did with my previous samples which is compare changes in microbes over time and between the locations. I was planning …

Additionally the binning generated fastq files which seems to be upsetting Galaxy greatly.

Do you know what the original data represented? Pacbio long reads are very different from amplicon reads. Is your original data uploaded to a public Galaxy server? If so or you can do that now, you could send me a direct message here with a share link to your history that contains that and the dat…

Hi just do more tutorials and keep asking questions about every problem. I was like you 8 mounth ago and now i run every project in my university which are related . @jennaj always answer and help with your problems. Cheers and wish you best

Wow, I am having heck even figuring out how to send a direct message. It doesn’t even offer me the option. The data is supposed to represent all the ribosomal RNA sequences in each water sample. So there are 16S and 18S sequences. I am supposed to be identifying the sequences with taxonomy, possibly…

Hi there, @jennaj were you able to find the history? I’m working a day job and doing this right now, so I am hopping back and forth. Sorry, for the delay.

Hi @n_griffith42 Sorry for the delay, missed your reply. I didn’t find an account registered under the same email address as used here at Galaxy Help at the Galaxy Main https://usegalaxy.org public server. I did, however, find an account that appears to be yours. I sent you a direct message – ple…

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I cannot figure out what to do with Fastq files that are already paired

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