I was having good success using the Mothur suite of tools for metagenomic work on Galaxy main before reaching the portion of taxonomy assignment in the protocol (I am using my own data but following the directions on the protocol as appropriate. https://galaxyproject.github.io/training-material/topics/metagenomics/tutorials/mothur-miseq-sop/tutorial.html#taxonomic-classification).
I am using a collection of datasets, one sequencing file broken down by barcodes with sequences stored in a separate collection based on the location. I have 142 total collections for the 142 separate barcodes that were used. I am using the SILVA reference taxonomy file.
My job has been in queue for 3 weeks now and the only information given is that the process is generating a list of jobs. This status has been consistent since the time I first submitted my job. The job doesn’t terminate, it just doesn’t progress.
Any ideas or information as to why this issue is occurring? Is this really an issue or am I just being impatient? This is my first post here so if more information is necessary please let me know and I will amend.