Hi,
Blockquote
I’m trying to go through your LEfSe platform on galaxy and I keep getting an error at the C) step.
This is the message I get: No differentially abundant features found in /home/usr/local/galaxy-dist/database/files/000/242/dataset_242547.dat
And it says that it is empty.
I join my data so you can take a look. I tried different things to format it as much as possible as your mouse data.
Welcome, @ayush_jain
What you have is a result, not necessary an “error”. The tool didn’t find any data that passed the algorithm for this tool so it didn’t have anything to write out. (And I agree, that could arguably be done better but it isn’t always possible for some tools).
It looks like you are working at the Biobakery server at Galaxy.bioBakery.org, and following their unique tools/protocol. The last time we had a question about it, the webserver was undergoing an update, and many people had problems. We won’t know the exact status here (sorry!). Please instead try contacting them directly since they host a dedicated forum.
If my guess is wrong and you are working at a different server, would you please clarify the URL so we can help to sort your question to the best place for help? It might be here! 
which means you can follow the help in the banner to learn how to share your history for review.