Metatranscriptomic analysis fails to determine pathwasy with HUMAnN

Hi @jenny.gamezroessler

Thanks for explaining more. As you probably know, KEGG is not independently available for free for non-academic use, so this impacts what we are able to publish on the public Galaxy servers. This topic has a few more details. → Request to enable KEGG and Pfam databases: licensing and KEGG - #2 by jennaj with the direct link here. → KEGG - Copyright and Disclaimer.

For your data, there is either actually no pathway information available for your hits, or there could be some technical issue that could be adjusted to better capture a result. We have another example in this tutorial step if you want to compare approaches. (Note that the downsampled training example didn’t have a pathway identified either!).

Would you like help reviewing? You are welcome to share your history back as a reply! :slight_smile: