Hello there! I am working with WGBS data and I want to extract the DMRs in a particular mutant. I used bwameth to align the sequencing data to mm10 genome. After that, I sorted the alignment file and used methyldackel to see the methylation status. I used the metilene tool to extract DMRs but this…

Thanks for asking! Yes I did, by removing the last two columns and first row (description) of the Methyldackel output. It then did the trick and Metilene took them with no problem! The weird thing is that I didn’t have this problem at all a year ago when I used the exact same files and process (usin…

Metilene tool usage

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jennaj June 20, 2023, 6:54pm 7

Xref: Metilene tool fails on ENCODE bigWigToBedGraph converted data - #4 by jennaj

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Metilene tool usage

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