How to build “Newick tree with study sequences placed amongst reference sequences” tree in galaxy for Picrusr2?
Welcome @Jeyamalar_Jeyanathan
Hopefully you found this already, but if you process your 16S data through the Mothur tools, you can use this tool.
- Tree.shared Generate a newick tree for dissimilarity among groups
- GTN Materials Search (query=mothur)
You can also use the PICRUSt2 tools directly. See the “Full” pipeline for the flow through the tools. You can run these individually, too. If you scroll down on the tool form, you’ll find links to tutorials → Home · picrust/picrust2 Wiki · GitHub
If you share what kind of data you have now and your analysis goals, we can try to help more! There are a few ways to do this. ![]()