Welcome @knagano
For a scientific discussion about aphla rarefaction curves, please see these tutorials at the Galaxy Training Network (GTN).
Mothur Hands-on: 16S Microbial Analysis with mothur (extended) / 16S Microbial Analysis with mothur (extended) / Microbiome- Phyloseq Hands-on: Building an amplicon sequence variant (ASV) table from 16S data using DADA2 / Building an amplicon sequence variant (ASV) table from 16S data using DADA2 / Microbiome
Then, all of the QIMME2 tools are hosted in Galaxy but do not have a Galaxy-specific tutorial. The protocol methods can instead be found at the original tool author website.
- alpha: Alpha diversity — QIIME 2 2024.10.1 documentation → Microbiome marker gene analysis with QIIME 2 - Microbiome marker gene analysis with QIIME 2
If you run into technical problems in Galaxy, you can ask questions here by sharing your history and we can try to help to solve the issue. You can also reach out at the QIMME2 forum for scientific questions about the underlying tools and methods protocol.
Hope this helps! 