Good morning,
I am a new user and managed to get my way round some tools for RNA sequencing analysis however, upon implementation of the RNA STAR tool (after all other queued jobs are complete) the RNA STAR jobs all go light blue paused and will not resume with the resume button. Please advise, I have been trying for a few days now and nothing has changed.
Many thanks
there should be some text displayed on those light blue datasets that would point to the underlying problem. Most likely something did not go well with some of the upstream steps.
If you can collect more information and post it here, we can certainly help.
Alternatively, you can send me a direct message with a link to your history and I can investigate things directly on the server.
Cheers,
Wolfgang
Hello, thank you. The message reads “Execution of this dataset’s job is paused because its input datasets are in an error state.” Any advice is greatly appreciated!
@wm75 I’m not sure how to DM on here but I will happily send you the history to help. Many thanks
Just click on my user icon, then Message 
@wm75 that just doesn’t seem to be an option for me! Would you be able to message me and I’ll try and DM back. Thank you!
Oh, might not be available to new users. I sent you a private message now.
Thanks for sharing your history with me @Charles_Cresswell , that helped a lot.
So the info message on the paused jobs is about an input dataset bein in error state. This refers to your dataset 81, which is an uploaded gtf. Confusingly, this dataset shows in green, but you set its format to gtf.gz.
Now when you go to one of the failed job’s Dataset Details (the i icon), you will see a red input dataset with a warning and it turns out the tool has tried (behind the scenes) to uncompress the input to uncompressed gtf, which failed because the input doesn’t look like a gzipped file.
Could it be that you uploaded the decompressed gtf and gave it the wrong format? If that’s the case, just change the format and things should work.
Thank you for the help, I will try reuploading as a regular gtf as that is how it is saved on my computer and then let you know! I appreciate your time.
Looks like it has worked! Thank you so much again for the help!