Scanpy FindVariableGenes

Two options:

Provide a file, one gene identifier per line, with genes that should never be active as HVGs. This simply makes sure that the boolean value for HVGs is false for all these genes after computing HVGs (–never-hv-genes-file)
Genes that should never be HVGs - optional

Provide a file, one gene identifier per line, with genes that should always be active as HVGs. This simply makes sure that the boolean value for HVGs is true for all these genes after computing HVGs (–always-hv-genes-file)

Did this two option work as the same that it exclude my gene list?how can Imakes sure that the boolean value for HVGs is true for my gene list after computing?

Welcome @xueraozhang321

Good catch! I see the typo on the Scanpy FindVariableGenes tool form.

For you question:

You will want to use the section option.

I’ll submit a change to get this fixed, thank you! :hammer_and_wrench: