Trouble whith demultiplexing tool of FROGS 4.1

[mlegeay]

Hi, I am using FROGS 4.1 Demultiplex read to assign my sequences to the right sample. I have created the barcode file as recquired :

The commands seems to work but when I look at the results I have a massive number of sequences not assign to any sample…

Has anyone ever had an experience like this? Does anyone have a solution ?

Thanks !

[jennaj]

Welcome, @mlegeay

I’m not sure what is going on but you can ask the scientists who run the server what they think. How to reach them is on the server homepage, or see the quote below for the direct link if my guess is correct.

These servers are all run by the same people. This is where you are working, yes?

• https://galaxy.pasteur.fr/
• https://usegalaxy.org/

@tcollins2011

Oh it sounds like you are having trouble accessing this tool. As this is probably an admin thing it is a question probably better asked directly to the usegalaxy.fr forum as they will have a better idea of how to help.

Alternative support forums

When working at certain Galaxy sites, questions are best asked at the places the scientists who run the server host discussions. They can also let you know if there happen to be any current technical problems that may be impacting your analysis.

UseGalaxy.fr → Galaxy - IFB Community Support

If you are not sure – check the URL at the top of your browser window. You can paste that back here and we can try to help more, too, in case my guess is wrong.

[mlegeay]

Hello,

Thank you very much for your answer I will reach the support !

Have a great day
Marie