Troubleshooting DexSeq

[jennaj]

Hi @ken67

These are the details about replicates that I know about:

@jennaj

Replicates will be required to use this tool and the other Bioconductor DE tools.

FAQ Extended Help for Differential Expression Analysis Tools

And, here is a summary from another person on our team, who captures the situation well, so am just quoting.

Yes, that is the problem.

See also DESeq2 without biol replicates - SEQanswers where Simon Anders and Michael Love chime in, the original authors of DESeq and DESeq2.

I think the best you can do is to normalize the counts and look at the fold changes, but none of that is really statistically sound.