i have the same problem with FEELnc

Hi @Eilyn_Mena Please share more details about the problem you are having, plus information about upstream steps. And are you also working at UseGalaxy.eu or somewhere else (URL?). [image] Use blastx on Galaxy could you describe to me your pipeline until now? Do you have raw RNA-seq data or ass…

Yes, i have assembled transcripts of soybean

Hi @Eilyn_Mena , I checked your error report, and it seems that the GTF file is empty. Could you confirm that? Regards

Thanks for your answer. I checked that, but both gtf file from my transcript and reference annotation are ok. I trait to run with data samples from galaxy but it not posible

Could you share your Galaxy history with me? My email is [Screenshot from 2022-04-03 16-52-09]. Regards

I shared vía galaxy and URL https://usegalaxy.eu/u/emena/h/171022

Dear, Do you detected any problem in my datasets

Hi @Eilyn_Mena , this seems to be the main error: Cannot read your input lncRNA GTF file 'out_feelnc/codpot//candidate_lncRNA.codpot.lncRNA.gtf I need to run some additional tests, but the problem could be an incompatibility with the GTF file generated by using GFF read. I will try to provide you …

thanks you, keep in touch regards, Eilyn

Galaxy Community Help

Troubleshooting use of FEELnc

transcriptomics, lncrna

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