Hello,
I am trying to remove the blacklisted regions from my ChIP-Seq datasets (Mapped regions to hg19 human genome, BAM files) using bedtools Intersect intervals (Galaxy Version 2.29.2), I used to do that for my previous datasets and it worked well, However, yesterday and today I am trying the same steps and I am getting this message:
The server could not complete the request. Please contact the Galaxy Team if this error persists. Error executing tool with id ‘toolshed.g2.bx.psu.edu/repos/iuc/bedtools/bedtools_intersectbed/2.29.2’: The effective dataset identifier consumed by object store [id] must be set before a path can be constructed.
{
“history_id”: “5afb961e3bb083e1”,
“tool_id”: “toolshed.g2.bx.psu.edu/repos/iuc/bedtools/bedtools_intersectbed/2.29.2”,
“tool_version”: “2.29.2”,
“inputs”: {
“inputA”: {
“values”: [
{
“id”: “11ac94870d0bb33af8237d1cea946e20”,
“hid”: 32,
“name”: “Veh-ARID1A-hTret-SJ77-Part2.Bowtie2 on data 19: alignments”,
“tags”: [],
“src”: “hda”,
“keep”: false
}
],
“batch”: false
},
“reduce_or_iterate|reduce_or_iterate_selector”: “iterate”,
“reduce_or_iterate|inputB”: {
“values”: [
{
“id”: “11ac94870d0bb33ab8996efdd404730b”,
“hid”: 31,
“name”: “Galaxy39-[Galaxy114-[hg19-blacklist.v2.bed.gz].bed].bed”,
“tags”: [],
“src”: “hda”,
“keep”: false
}
],
“batch”: false
},
“strand”: “”,
“overlap_mode”: null,
“split”: “false”,
“fraction_cond|fraction_select”: “default”,
“invert”: “true”,
“once”: “false”,
“count”: “false”,
“bed”: “false”,
“sorted”: “false”,
“header”: “false”
}
}
Could you please help,
Thank you very much!
Sham