Which COI primers are suitable for mylabris pustulata DNA barcoding?

Hina_Bibi · September 26, 2026, 9:59am

I am planning COI DNA barcoding of Mylabris pustulata and would appreciate advice from researchers working with Mylabris or Meloidae.

I am considering either the universal COI primers LCO1490/HCO2198 or a universal forward primer with the Meloidae-specific reverse primer:

CO1MelZ2R: 5′-GGGTCAAAGAARGATGTATT-3′

CO1MelZ2R has been reported for Meloidae and used with a universal forward primer.

I would particularly like to know whether CO1MelZ2R + a universal forward primer has been used successfully for Mylabris, especially M. pustulata.

I would also appreciate references to any other published COI primers that have been used or recommended specifically for M. pustulata.

Any practical experience regarding PCR success, amplification quality, and obtaining good-quality COI sequences would be very helpful.

Thank you!

jennaj · October 1, 2026, 12:13am

Most of the people that visit this forum are seeking help with using the https://galaxyproject.org platform.

To reach the scientists working with your species, I would suggest trying a scientific forum like Biostars.org instead.

Hope this helps!