Hi @H1889
Yes, this can happen with certain combinations of settings. We have some troubleshooting in this similar topics → #busco
This is a fresh testing history. I’m guessing that your options were set up like the first example run that errored.
So, instead, try one of the other combinations. Some can be unexpected. But if a database “isn’t available” in the error message, that means the underlying tool cannot process data that way.
GTN Tutorials for Genome Annotation / Tutorial List