Hi Jenna, I am having the exact same error as posted. I Have an abundance of fast5 Files that return the same result. Was there an eventual solution to this formatting issue? Would be more than happy to share my history if this helps? Thanks! Jesse

[image] jessemartin: The original post said there was some formatting issues, could this also be the problem with my data? Yes, this is likely a problem for you as well if tools cannot read your files. If the data is compressed, then that compression format should be included on the dataset …

Extracting reads from fast5 format

jennaj December 21, 2022, 12:22am 2

Hi @jessemartin

I just reran the two test tools in this history, and both are still functional. Maybe that helps?

Topic		Replies	Views
Issue with .fast5 files from nonopore sequencing upload	1	19	July 25, 2024
Nanopolish running upload , single-cell	3	235	August 25, 2023
Will Nanopore-related tools be updated to accept pod5 files? usegalaxy.eu support single-cell , variant-analysis	2	145	July 8, 2024
Get Longest Read?? single-cell	1	273	February 28, 2023
Error in tools .. feb 3 usegalaxy.org support upload , troubleshooting , quality-control	2	237	February 10, 2024

Extracting reads from fast5 format

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