failure importing data with tximport with error information “Missing filters: deflate”

hello, I am new to bioinformatics and Galaxy, so maybe my question is kind of naive. I’m reading RNAseq count data generated from kallito using tximport:

humandb<- EnsDb.Hsapiens.v75
tran.human<- transcripts(humandb, return.type = “DataFrame”)
tran.human<- tran.human[, c(“tx_id”, “gene_id”)]

gene.human<- genes(humandb, return.type = “DataFrame”)
gene.human<- gene.human[, c(“gene_id”, “symbol”)]

tx2genes.human <- merge(tran.human, gene.human, by = “gene_id”)
tx2genes <- as.data.frame(tx2genes.human[, 2:3])
colnames(tx2genes)[1]<-“target_id”
txi.kallisto <- tximport(files, type = “kallisto”, tx2gene = tx2genes, ignoreTxVersion = TRUE)

loadname <- c(“ERR2814755”,“ERR2814756”)
name<-candidate$sample
files <- file.path(path,loadname, “abundance.h5”)
names(files) <- loadname

txi.kallisto <- tximport(files, type = “kallisto”, tx2gene = tx2genes, ignoreTxVersion = TRUE)

it used to work, but after 10.29, when I try it again without any change, it gave the error information as follows:

Error: Unable to read dataset.
Not all required filters available.
Missing filters: deflate

what can I do……:rofl:
R 3.6 is running under the Linux system

1 Like