I was trying to assemble a bacterial genome of about 2.1 Mb using flye. These are long reads produced with nanopore that I decompressed and then filtered by length; I have used this protocol several times without any problems. Even with genomes made in the same sequencing run.
Unfortunately, this particular sample always gives me an error. I have tried both on galaxy.eu and galaxy.org.
It sounds like you’ve done all the cross-testing we would usually suggest, so that part is great!
To investigate this more, it would be helpful to see the messages on the expanded red datasets and the full Standard Output/Error logs. You can click on those sections and each will expand for a screenshot.
I would also be curious about the fitlong job, e.g. what it did or didn’t do. That is more complicated to screenshot. But have you reviewed how that differs for this sample versus the others that did assemble? Any observations?
If you want to set the history to a shared state and post that link back it can be more helpful when troubleshooting, but you decide.