FreeBays Error for variant calling

Please I receive this message for my analysis: ERROR(freebayes): could not find SM: in @RG tag @RG ID:s1_r1 PL:ILLUMINA

Please any help

Hi @Sofiane_Abdelhamid

The error points on missing sample name tag in read group. Have you added read group(s) during mapping step or user AddOrReplaceReadGroup tool? Check the job setup of this step (when read group(s) were added and make sure you added all required tags. You probably can fix the read group using AddOrReplaceReadGroup.

Kind regards,

Igor

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