Welcome @Latif_khan_shamozai
If you are not following the tutorial yet, this is where to find it → Hands-on: Identifying Mycorrhizal Fungi from ITS2 sequencing using LotuS2 / Identifying Mycorrhizal Fungi from ITS2 sequencing using LotuS2 / Microbiome
Then, for us to help more, we’ll need to see more the job details. The banner at this forum explains how to generate the history share link you can post back here in a reply. Also here directly → How to get faster help with your question
Let’s start there, thanks! 