I attempted to run LOTUS2 on the Galaxy server multiple times, but the job failed

[Latif_khan_shamozai]

I attempted to run LOTUS2 on the Galaxy server multiple times, but the job failed each time with an error. I have carefully followed the required steps, ensuring that:

• My data is correctly formatted as paired-end ITS sequences.
• I created a list of paired-end reads.
• I provided a mapping file.
• I specified primers as required by the tool.
  
  

[jennaj]

Welcome @Latif_khan_shamozai

If you are not following the tutorial yet, this is where to find it → Hands-on: Identifying Mycorrhizal Fungi from ITS2 sequencing using LotuS2 / Identifying Mycorrhizal Fungi from ITS2 sequencing using LotuS2 / Microbiome

Then, for us to help more, we’ll need to see more the job details. The banner at this forum explains how to generate the history share link you can post back here in a reply. Also here directly → How to get faster help with your question

Let’s start there, thanks!