Normalizing RNA-seq with RUVIII - PRPS

Hello - does anyone have experience with RNA-seq normalization using RUVIII PRPS? * PMCID: [PMC9849124] Removing unwanted variation from large-scale RNA sequencing data with PRPS - PMC). Have an RNA-seq dataset that didn’t show a ton of robust changes and manuscript reviewer suggested I use RUVIII to normalize. Disclaimer - not a coder and lean heavily on Galaxy tools.

Welcome, @olddog1

The public UseGalaxy servers do host this tool.

I also think the search terms to find this tool could be improved. I included that request to the developers in the ticket above as a small note. Maybe we can improve that in the future!

Very glad the public Galaxy servers and hosted tools are working out for you! :slight_smile: And, thank you for including the publication as a reference, super helpful. Please let us know if this is enough.

Much Appreciated! I’ll let you know.

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