Hello - does anyone have experience with RNA-seq normalization using RUVIII PRPS? * PMCID: [PMC9849124] Removing unwanted variation from large-scale RNA sequencing data with PRPS - PMC). Have an RNA-seq dataset that didn’t show a ton of robust changes and manuscript reviewer suggested I use RUVIII to normalize. Disclaimer - not a coder and lean heavily on Galaxy tools.
Welcome, @olddog1
The public UseGalaxy servers do host this tool.
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Remove Unwanted Variation from RNA-seq data
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link to the tool at the UseGalaxy.eu server, and it is also available at UseGalaxy.org (link)
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I didn’t find it at UseGalaxy.org.au, yet, but made a request here.
I also think the search terms to find this tool could be improved. I included that request to the developers in the ticket above as a small note. Maybe we can improve that in the future!
Very glad the public Galaxy servers and hosted tools are working out for you! And, thank you for including the publication as a reference, super helpful. Please let us know if this is enough.
Much Appreciated! I’ll let you know.
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