Hi, In untrimmed fast QC it shows me errors : Per sequence GC content = ! Sequence Length Distribution = ! Sequence Duplication Levels = ! So how i can the improve the duplication level ?? This one is before trimming: [image] This one is after trrimming: [image]

regd fastqc ouput

gallardoalba August 26, 2022, 2:57pm 2

Hi @Thara_Dharanidhar,
I suggest you to remove the first 10 nucleotides form the 5’ end by using PRINSEQ.

Regards

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